CALCA and pancreatic neoplasm: To test this in the context of pancreas tumor initiation, we performed the Assay for Transposase-Accessible Chromatin followed by high-throughput sequencing (ATAC-seq) (Buenrostro et al., 2013) on mKate2+GFP+ cells sorted from KC-RIK-shRen and shArid1a mice after 5 days on dox, as in our transcriptional profiling experiments.