In this study, we determined the frequency and phenotype of wt sequence p53 peptide-specific T cells in the peripheral circulation of HLA-A*0201+ patients with HNSCC by multicolor flow cytometry using HLA-A*0201 tetramers (tet) complexed with wt sequence p53264–272 or p53149–157 peptides, referred to as tet264–272 and tet149–157, respectively. The gene discussed is TP53; the disease is head and neck squamous cell carcinoma.