To further validate the functional regulation of metastasis by TMEM106B, its expression was stably inhibited by shRNAs targeting different segments of the TMEM106B mRNA in the highly metastatic KrasLA1/+;TP53R172HΔG (KP)7,36 mouse lung cancer cells, 344SQ and 344LN (TMEM106B-sh1–sh3; Fig. 2a, b and Supplementary Figure 1A), and human A549 cells (Supplementary Figure 1E), all of which have high endogenous TMEM106B levels (Supplementary Figure 1D). This evidence concerns the gene TMEM106B and lung carcinoma.