TMEM106B and lung cancer: We observed that in either scenario there was no significant change in TMEM106B, at the mRNA (Supplementary Figure 4F) or protein (Supplementary Figure 4G) level, indicating that TMEM106B expression was not regulated by TFEB. Taken altogether, these results suggest that TMEM106B expression in lung cancer cells results in increased lysosome size and enhanced activity of TFEB, thereby modulating expression of the CLEAR genes.