TMEM106B and lung cancer: To further validate the functional regulation of metastasis by TMEM106B, its expression was stably inhibited by shRNAs targeting different segments of the TMEM106B mRNA in the highly metastatic KrasLA1/+;TP53R172HΔG (KP)7,36 mouse lung cancer cells, 344SQ and 344LN (TMEM106B-sh1–sh3; Fig. 2a, b and Supplementary Figure 1A), and human A549 cells (Supplementary Figure 1E), all of which have high endogenous TMEM106B levels (Supplementary Figure 1D).