To analyze AKT isoform-specific expression and activation, we first characterized AKT specific antibodies using a set of parental and AKT knockout HCT116 colon cancer cells (AKT1−/−, AKT2−/−, and AKT1/AKT2 DKO) [22]. The gene discussed is AKT2; the disease is malignant colon neoplasm.