We noted that the majority of the studies using IgM ELISA [8,33,35,36,38,44,54,56,60,68,71,76,82,87,108,119,120,120,121,125,128,130–133,139–141,143], NS1 tests [149] and/or RT-PCR [35,60,76,108,118,141,159,160] were conducted in an outbreak or post-outbreak context (p<0.001), which is not surprising because these tests are used for detecting acute and/or recent infections. The gene discussed is CD40LG; the disease is infection.