MICA and HIV-1 infection: Latent HIV-1 infection was established in primary resting CD4+ T cells pre-treated with CCL19 according to a previously reported protocol (see Materials and Methods), then cells were exposed for 72 h to 1 μM PRO, 10 mM BRY, or, as controls, activated with 10 μg/ml of PHA or not stimulated (ns), prior analysis of intracellular p24 and cell-surface MICA/B and ULBP2 expression by two-color flow cytometry (representative and summary data with four donors are shown in Figures 4A–D).