Purified CD8 T-cells from an HLA A*02:01+ type I diabetes patient were stimulated with peptides from CMV, GAD65, IGRP, IA-2, and the insulin-β chain (Table 2) to create T-cell lines for testing with standard and optimized tetramer staining protocols (Figure 2A) (see Figure S1 in Supplementary Material for optimized protocol schematic). Here, GAD2 is linked to type 1 diabetes mellitus.