In addition, the in our samples altered regions 20p12 and 20p13 hold the known tumor suppressor genes Phospholipase C Beta 1 and 4 (PLCB1, PLCB4) and Casein Kinase 2 Alpha 1 (CSNK2A1), all previously described to be associated with advanced tumor stages and bad outcome in different cancers but not yet in iCCA37–39. This evidence concerns the gene PLCB4 and cancer.