As an example, the TIMP2 and ITIH5 genes showed decreased 5hmC in gene bodies in bladder cancer compared with the normal bladder samples, and hMeDip-qPCR/MeDip-qPCR verified the increase in 5hmC and a relative decrease in 5mC (Fig. 2e). The gene discussed is ITIH5; the disease is urinary bladder carcinoma.