We calculated the accuracy of ZIKV IgM- and IgG-ELISA tests, with respective 95% confidence intervals, using the samples from RT-PCR-confirmed cases of ZIKV infection as the reference group of positives for sensitivity estimation, and the samples from RT-PCR-confirmed dengue cases, as well as blood donors as reference groups of negatives for specificity estimation. This evidence concerns the gene CD40LG and Zika virus infectious disease.