After knocking down vimentin in MDA-MB-231 and Hs578T breast cancer cells, which highly express endogenous vimentin, liprin-α1 was not localized near the cell edge or after the leading edge as in control cells, although there were no significant changes at the protein expression levels in these breast cancer cell lines (Additional file 10: Figure S7D-E). This evidence concerns the gene VIM and breast carcinoma.