ERBB2 and breast neoplasm: We analyzed 18 HER2+ breast tumors using a combination of mate-pair genomic sequencing (MPseq) [21–23], RNA sequence analysis (RNAseq), and NanoString 3D Biology TM to assess the extent to which large chromosomal alterations both within and outside of chr 17q12 are observed and are associated with ERBB2 amplification and overexpression.