Other than expected, none of the metabolic (adiponectin, resistin, leptin) and inflammatory factors (CRP, TNF-α, IL-6, IL-8 and IFN-γ), which have been proposed as potential circulating markers of NAFLD [24, 25], were significantly associated with liver fat content (%) and NAFLD in our study. This evidence concerns the gene TNF and metabolic dysfunction-associated steatotic liver disease.