To detect the expression pattern of Wnt4 in the cisplatin-induced AKI model, we co-stained Wnt4 with aquaporin-1 (AQP-1, a proximal tubular marker) and thiazide-sensitive NaCl cotransporter (NCCT, a distal tubular marker) and compared the localization with Kim-1. The gene discussed is SLC12A3; the disease is acute kidney injury.