To evaluate the effect of resveratrol and/or 5-FU on TNF-β-induced invasion capacity of CRC cells in a 3D inflammatory tumor microenvironment, HCT116 and HCT116R cells (1 × 106/mL) were cultured in an alginate-based matrix, treated as described in detail in Material and Methods and the capacity of migration and invasion was determined through evaluation of colony-formation with toluidine blue staining after 10 days. This evidence concerns the gene LTA and neoplasm.