We extended the MHC class I binding prediction analysis to include, in addition to CALR, some other mutations found recurrently in hematological malignancies e.g. FBXW7, TP53, and MYD88. These putative neoepitopes were used to generate a panel of pMHC multimers, to enable the isolation of neoepitope-specific CD8+ T cells from the T cell pool of readily available healthy human donor PBMC. Here, CD8A is linked to hematologic disorder.