Secondly, we used a MHC class I binding peptide prediction algorithm to identify potential mCALR neoepitopes presented by common European Caucasoid HLA class I alleles, generated pMHC multimers encoding these predicted CALR peptides, and subsequently used combinatorial-encoded mCALR multimer staining to screen mCALR+ MPN patients for CD8+ mCALR T cell responses. The gene discussed is CD8A; the disease is myeloproliferative neoplasm.