To explore the biological role of PVT1 in PDA autophagy, we first performed ectopic expression of PVT1 in Capan-2 and MIA PaCa-2 cells by transfected with full-length of PVT1 cDNA, and knocked down PVT1 expression in SW1990 and HPAF-II cells using two small hairpin RNAs (shRNAs). This evidence concerns the gene PVT1 and Patent ductus arteriosus.