To understand the dynamics of the molecular changes related to apoptosis and the phenotypic changes observed in the cancer cells, cleaved caspase-3, caspase-8, caspase-9, and cleaved-PARP, CA IX protein level, γ-H2AX, pHi, pHe, lactate level change, MMP, ROS activity, cell cycle distribution, amino acid metabolism and gene expression analyses were used. This evidence concerns the gene CA9 and cancer.