To further investigate the role of TdEno in the production of anti-ENO1 antibodies and the progression of periodontitis, mice that received oral gavage of P. gingivalis alone (Pg), subcutaneous immunization with TdEno alone (TdEno), or both P. gingivalis oral gavage and TdEno immunization (Pg+ TdEno) were compared with the sham group (Figure 3(a)). Here, ENO1 is linked to periodontitis.