Survivin mRNA, which is responsible for survivin overexpression in all of the most common human malignancies, has become a significant tumor marker.34,35 Highly sensitive monitoring of survivin mRNA transcripts is of great significance for early disease detection and screening.36–40 In this study, we used single asymmetrically modified plasmonic AuNPs as independent invisible monomer probes for monitoring genetic material-survivin mRNA in living cells (Scheme 1). The gene discussed is BIRC5; the disease is neoplasm.