Our data suggest that (1) suppression of IL12B transcription by ovarian carcinoma ascites acts immediately and on differentiated macrophages, and it is reversible upon ascites withdrawal; (2) although ascites impinges on REL and p65 nuclear translocation, binding of these factors to chromatin is not diminished; and (3) IL-10 can only partially recapitulate suppression. Here, IL12B is linked to ovarian carcinoma.