To clarify whether long-term exposure to ascites has lasting effects on the capacity of MDMs to express IL12B, monocytes from three healthy donors were differentiated either in R5 or in each of five different cell-free ascites samples from OC patients for 12 days (long-term exposure) in order to mimick the cellular state after differentiation in the ovarian tumor microenvironment. Here, IL12B is linked to ovarian neoplasm.