coli isolates including 42 strains of the HUSEC collection (Mellmann et al., 2008), 83 previously characterized clinical isolates obtained from the German National Consulting Laboratory for HUS-associated E. coli as well as the K-12 lab strain MG1655 as a non-pathogenic control and the O104:H4 enteroaggregative E. coli (EAEC) strain 55989 to distinguish between stx2-positive and stx2-negative O104:H4 EAEC (Checkpoint 4 in Figure 1, Table 3 and Supplementary Table S5). Here, STX2 is linked to hemolytic-uremic syndrome.