To determine whether TSPf also induced AML cell apoptosis, two more AML cell lines KG-1 and HT-93, in addition to K562 and HL-60, were incubated with TSPf from 0 to 8 μg/ml for 24 h before being applied for Annexin V-FITC and PI staining and the analyzed by a flow cytometer. This evidence concerns the gene ANXA5 and acute myeloid leukemia.