With the improvement of electronic medical record networking system and the development of mobile laboratory, biomarkers which had been proved to be correlated with ischemic stroke, such as D-dimer, Cystatin C (Zou et al., 2017) and low-density Lipoprotein (Weng et al., 2018) can be added in the ANN model. The gene discussed is CST3; the disease is ischemic stroke.