We treated p18−/−;Brca1MGKO and SUM149 tumor cells with or without E2 and found that E2 stimulated expression of p-Akt, p-4Ebp1, p-mTor, and p-Gsk3β in all time points tested from 2 to 144 h (Fig. 3e, g, Fig. 5c, Fig. 6a and Additional file 6: Figure S6A, B, E). Here, AKT1 is linked to neoplasm.