The great majority of TMA samples mapped to one or a few discrete locations in the t-SNE projection (compare normal kidney tissue - KI1, low-grade tumors - KI2, and high-grade tumors – KI3; Figure 10C), although ovarian cancers were scattered across the t-SNE projection (Figure 10D); overall, there was no separation between normal tissue and tumors regardless of grade (Figure 10E). This evidence concerns the gene TNFRSF8 and ovarian cancer.