AKT1 and cancer: Through combination of RNA sequencing data and biological in vitro/in vivo results, we hypothesized the signalling pathways involved in cell cycle progression, which were shown in Figure 5A. We believed that after Gonearrestide bound with the cancer cell membrane, cationic Gonearrestide could affect PIP3 by electrostatic attraction to its anionic trisphosphate group, resulting in the inhibition of the Akt pathway.