Oxidative stress and inflammation are hallmarks of endothelial senescence.52, 53 Kallistatin administration has been shown to attenuate vascular injury and organ damage and ageing, in conjunction with reduced oxidative stress and inflammation in animal models with hypertension, arthritis, diabetes and sepsis.37, 38, 39, 40, 48, 54 In this study, we showed that kallistatin inhibited H2O2‐induced oxidative stress and pro‐inflammatory gene expression, including VCAM‐1, ICAM‐1 and IL‐6, in human endothelial cells. Here, VCAM1 is linked to diabetes mellitus.