Recently, in a mouse model fed with a high-fat diet for 6 weeks, the reduction of the eNOS activity was demonstrated in the early stages of NAFLD, with the result of reducing NO bioavailability, together with an increased oxidative stress and cyclooxygenase activity, resulting in an increase of hepatic vascular resistance unrelated to inflammation or fibrosis [104]. The gene discussed is NOS3; the disease is metabolic dysfunction-associated steatotic liver disease.