TP53 and cancer: Functional annotation of DEmRNAs showed that the most enriched biological processes were skeletal system development, ossification, positive regulation of cartilage development, intramembranous ossification, osteoclast differentiation, etc. Pathways in cancer, osteoclast differentiation, rheumatoid arthritis, Toll-like receptor signaling pathway, p53 signaling pathway, Wnt signaling pathway, Jak-STAT signaling pathway may be closely involved in OS tumorigenesis.