Besides, key glycolysis enzymes, such as pyruvate kinase M2 (PKM2; also known as PKM), LDH and glucose-6-phosphate dehydrogenase (G6PDH) have increased activity in breast CSCs, while treatment with 2-deoxyglucose (2DG), a glucose analogue that inhibits hexokinase 2 (HK2), preferentially decreases the proliferation of these cells compared with mature cancer cells, showing that glycolysis is essential for breast CSCs (Ciavardelli et al., 2014). This evidence concerns the gene PKM and cancer.