NANOG and hepatocellular carcinoma: In a mouse model of hepatocellular carcinoma, CSCs expressing Nanog (Box 1) have increased glycolytic activity and fatty acid oxidation (FAO), decreased mitochondrial respiration – owing to cytochrome C oxidase subunit 6A2 (Cox6a2) repression, and inhibition of ROS generation (Fig. 1A), suggesting that a defined metabolic pattern regulates stemness in this model (Chen et al., 2016).