Briefly, this integrated analysis of genomewide DNA methylation and gene expression data identified a group of novel gene signatures (ALX3, B4GALNT1, CTHRC1, DLX5, EMX1, IRX3, OTX1, SIX2, TLX1, VASH2, ZIC2, ZIC4, ZIC5, and ZNF695), which may be regulated by DNA methylation in HCC. The gene discussed is SIX2; the disease is hepatocellular carcinoma.