To confirm and extend the observations regarding the presence of HK1−HK2+ liver cancers and to determine whether HK1−HK2+ subsets exist in cancers from other tissues of origin, we examined the frequencies of HK1−HK2+ and HK1+HK2+ subsets in established cancer cell lines of the Cancer Cell Line Encyclopedia (CCLE) collection [16]. This evidence concerns the gene HK2 and liver cancer.