However, HK1+HK2+ cancer cell lines from a variety of cancers (liver, breast, colon, prostate, ovary, lung), including the lung cancer H460 cell line, are resistant to HK2 silencing alone (Fig. 2a–d) or the synthetic lethality of the shHK2/DPI/PER (Additional file 1: Figure S5C). This evidence concerns the gene HK1 and lung carcinoma.