We find neither cell proliferation (Fig. 2a) nor colony formation (Fig. 2b) are affected by shHK2DOX HK2 inhibition for HK1+HK2+ HLF and JHH6 liver cancer cells, suggesting pharmacologic HK2 inhibition will not have therapeutic efficacy for HK1+HK2+ liver cancers. Here, HK2 is linked to liver cancer.