DOX-induced shHK2 expression in the HK1−HK2+ H460 cells suppressed colony formation but had no effect on isogenic HK1+HK2+ H460 cell colony formation (Fig. 2e); conversion of an HK1+HK2+ cancer cell to an isogenic HK1−HK2+ cancer cell has no effect on cell growth or proliferation but converts cells resistant to shHK2-mediated inhibition of proliferation or colony formation to cells sensitive to shHK2 inhibition. Here, HK2 is linked to cancer.