IL-10 is a well-known immunosuppressive cytokine, which is secreted by tumour cells and Tregs among other immune cells and has an important role in tumour immunosurveillance.60 On the other hand, IFNγ is a pleiotropic molecule with antitumour effects, which is produced primarily by cytotoxic CD8+ T lymphocytes and NK cells.61 H4R stimulation enhanced IL-10 while decreased IFNγ levels in tumour conditioned medium of WT mice, suggesting an immunosuppressive role of H4R in the context of 4T1 breast cancer model. This evidence concerns the gene IFNG and neoplasm.