To determine the effects of PDBu and HGF on invadopodia formation, as well as investigating the expression of cortactin in lung cancer cells, A549 and H1299 cells were stimulated with PDBu or HGF, or were unstimulated, and the changes in cell morphology and invadopodia were recorded with immunofluorescence assays. This evidence concerns the gene CTTN and lung carcinoma.