A comparison of the incubation period and PK-resistant PrPSc molecular profile observed in tg338 mice inoculated with either a sample of the original PG127 sheep scrapie inoculum, or this material passaged in VRQ Drosophila, suggested that mammalian prion strain properties were unaltered following propagation in PrP transgenic Drosophila. To confirm this was the case we propagated three distinct ovine prion strains in PrP transgenic Drosophila prior to their re-isolation in tg338 mice. Here, PRNP is linked to scrapie.