We used Galene to image the activation of Src, a key regulator of metastasis in PC (Erami et al., 2016; Evans et al., 2012; Nobis et al., 2013; Vennin et al., 2017), via FLIM-FRET in cancer cells arriving and attaching in the liver in an intrasplenic metastasis model of pancreatic cancer using live longitudinal imaging with titanium windows. The gene discussed is SRC; the disease is familial pancreatic carcinoma.