Active enhancer profiling, using the H3K27ac mark, coupled with whole-transcriptome analysis of UC and non-IBD organoids, highlighted multiple genes whose expression is altered in UC organoids and that are associated with pathways known to be activated in colitic disease, genes such as LYZ and S100P. Other genes, including NPSR1, were identified as new disease signatures and may be involved in disease pathogenesis. Here, NPSR1 is linked to inflammatory bowel disease.