CASP3 and tarsal-carpal coalition syndrome: To determine the effects and mechanisms of anthracyclines-induced apoptosis in human TCC cells, we measured the activation of caspase-3/7 and cleavage of PARP in RT4, SW780, 5637, UM-UC-3, T-24, J82 and TCCSUP cells 24 hours after treatment with 1 μM Dox, 10 μM AD 312, and 1 μM AD 198.