2006; Nunez et al. 2007, 2008; Hong et al. 2009), total body fat gain (Nunez et al. 2007), and impaired insulin sensitivity and glucose regulation (Kumagai et al. 1993). A HFD combined with OVX further increases weight gain in female rodents compared to OVX alone (Yakar et al. 2006; Nunez et al. 2007, 2008), and the protection from T2D that high‐fat fed female rodents demonstrate over male rodents diminishes when combining the HFD with OVX (Hong et al. 2009) or after the rats become acyclic (Gomez‐Perez et al. 2008). Here, INS is linked to type 2 diabetes mellitus.