Results also demonstrated that Nfatc4 increases gene expression of Pparg, Tcf7l2, and Wfs1 which are genes already reported to be associated with T2D as well as activation of the Wnt pathway as predicted by systems genetics approach that is subsequently validated in vitro.28 However, silencing Nfatc4 also appears to have a tendency to inhibit osteopontin (Opn). This evidence concerns the gene TCF7L2 and type 2 diabetes mellitus.