LGR5 and cancer: Then, the drug combination treatment blocked aberrant Wnt signaling in DLD1 cells bearing APC mutation, with no effect in non-cancer cell lines (C2C12 and MRC5), manifested by strong decrease of expression of Wnt target genes, AXIN2 and LGR5. LGR5 is a colon and lung cancer stem cell marker55,56, and its expression reflects stemness-like properties of cancer cells (Fig. 5e and Supplementary Fig. S9d).