EP300 and infection: To determine if the interaction of p300 with the E1A activation domain influences E2, E3, and E4 transcription via acetylation of histone H3 during infection, we also performed ChIP-seq for H3K18ac, H3K27ac, and H3K9ac in virus-infected HBTECs expressing equal concentrations of wt and DM E1A protein at 12 and 18 h p.i. We observed dramatic decreases in H3K18/27ac peaks at the E2early, E3, and E4 promoters in cells expressing DM E1A compared to wt E1A (Fig. 8).