The virtual absence of H3K18/27ac at the E2early promoter following infection with the DM correlated with an absence of TBP and Pol II association, suggesting that H3K18/27ac by p300/CBP is required for E1A stimulation of Pol II preinitiation complex assembly in vivo at this promoter (Fig. 8). This evidence concerns the gene DHTKD1 and infection.