EGFR and large cell carcinoma: We first examined the EGF-induced endocytic degradation of EGFR in a panel of NSCLC cell lines, which includes all major histologic subtypes: adenocarcinoma (A549, H1299, HCC827, H1650, and H1975), large cell carcinoma (H460), and squamous cell carcinoma (H226 and SK-MES-1).