A dosage of 1 mg/kg 3AOA for our study was determined based on previous in vivo experiments we conducted using a heterotopic CT-26 colon carcinoma animal model, in which an intraperitoneal injection of 3AOA at a dosage of 1 mg/kg successfully inhibited the tumor growth that was induced by recombinant angiopoietin-1. This evidence concerns the gene ANGPT1 and neoplasm.