CD80 and neoplasm: Here, we have shown that G3 CAR T-cells, cocultured with CD32-80-137L-EGFRVIIIΔ654 aAPC cells (CD32, CD80, 41BBL, and EGFRvIII) can survive at day 90, provide enhanced anti-tumor activity, and able to destroy tumor stroma with single treatment of 25,000 CAR T-cells.