IFNG and neoplasm: When pre-cultured on CD32-80-137L aAPC (Fig 5A), both G1 (F2,2 = 8157, p = .0002) and G3 CAR T-cells (F2,2 = 8717, p = .0002)showed robust IFN-γ expression (>3000 pg/125,000 cells/24 hours) compared to delζ CAR T-cells upon co-incubation with EGFRvIII positive tumor cells in a cytolysis assay.