Patients with OCT4high/LEF1high tumors had a poorer prognosis and more aberrant clinicopathological features than the high/low and low/low expression groups, and Spearman's rank correlation results showed that the aberrant expression of LEF1 was positively associated with the overexpression of OCT4 in ESCC. The gene discussed is POU5F1; the disease is esophageal squamous cell carcinoma.