Eight of the 13 ALFRED genes with a nominally significant association between RDGVs and AI in at least one cancer type in the ALFRED analysis (P < 0.05; Fig. 2d and Supplementary Data 5) also had an enrichment of RDGVs in a matched cancer type compared to in controls (P < 0.05, Fig. 3d; Supplementary Data 6): ATM in colon and rectum adenocarcinoma (COADREAD), lung adenocarcinoma (LUAD) and in PRAD, NSD1 in OV, and TPCN2 in uterine corpus endometrial carcinoma (UCEC). This evidence concerns the gene NSD1 and rectum adenocarcinoma.