Tumours were fixed and stored as paraffin-embedded tissue to study the tumour histology with haematoxylin and eosin staining (H&E) and by immunohistochemical analysis using antibodies against the myoepithelial cell markers alpha-smooth muscle actin (αSMA) and p63 (Additional file 5: Figure S2A). This evidence concerns the gene ACTA1 and neoplasm.