Chemoresistance is an essential characteristic of CSC.32 To investigate whether AFAP1‐AS1 functions as a dominant determinant of chemoresistance in laryngeal carcinoma cells, we treated AFAP1‐AS1‐silenced HEp‐2 cells with 4 μmol L−1 cisplatin and analysed AFAP1‐AS1 expression at different time‐points. This evidence concerns the gene AFAP1-AS1 and laryngeal carcinoma.