The further use of DIANA Tools and a number of cell biology assays allowed us to determine conclusively that miR‐320a targets RBPJ and that AFAP1‐AS1 increases RBPJ expression by negatively regulating miR‐320a, thereby giving rise to laryngeal carcinoma's stemness and chemoresistance. The gene discussed is RBPJ; the disease is laryngeal carcinoma.