These genes were used as markers to indicate the stemness of CSC.29, 30, 31 To further explore AFAP1‐AS1 function in laryngeal carcinoma cell development, we knocked down AFAP1‐AS1 expression using siRNA transfection and confirmed silencing with qRT‐PCR (Figure 1D). The gene discussed is PTGDR; the disease is laryngeal carcinoma.