After determining that AFAP1‐AS1 promotes cancer cell stemness and cisplatin chemoresistance, we used the DIANA Tools gene targeting prediction algorithm, luciferase reporter assays and CCK8 cell viability assays to demonstrate that miR‐320a overexpression reduces laryngeal carcinoma cell stemness while increasing chemosensitivity to cisplatin. Here, AFAP1 is linked to laryngeal carcinoma.